Detailed Monoclonal Antibodies Development
Program (Pay for Success program) View this page in PDF file format(opens in a new window)
Phase I. Immunization and test bleeding: 1.5-2.0 months (1,500 USD)
Immunization - 3 Balb/c mice are immunized and boosted using a Adar
Biotech's immunization protocol.
Screening - Tail bleedings are screened by direct ELISA to determine
antibody titer.
Evaluation - The ELISA results are sent to the customer for evaluation
Phase II. Fusion, clone selection and screening: 1 month (3,400 USD)
Fusion - Splenocyte fusion to Mouse Myeloma cell line is performed on
the mouse which responded the best to the immunization. 5-6 96-well
microplates are commonly prepared.
Selection and screenings - Are done for isolating antigen specific- IgG
secreting hybridomas We send the data to the customers and chose
with the customer the best 2-4 clones.
Phase III. subcloning and delivery: 1.0-1.5 months (2,000 USD)
Subcloning - The 2-4 clones selected with the customer are subcloned
by limiting dilution cloning.
Delivery- 4 vials of hybridoma cells from each clone and 5 ml of culture
supernatants from each clone are delivered to the customer.
Evaluation - The ELISA results are sent to the customer for evaluation
Total quote: 6,900 USD for the three phases
The Pay for Success Program
Phase I. Immunization and test bleeding: 1.5-2.0 months (1,500 USD)
Mandatory payment
Phase II. Fusion, clone selection and screening: 1 month (3,400 USD)
Phase III. subcloning and delivery: 1.0-1.5 months (2,000 USD)
Pay for success: Delivery of 2 hybridoma clones or more, secreting IgG
class antibodies to yield OD>0.6 in direct ELISA, on antigen coated
plates.